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. 2016 May 11;6:25729. doi: 10.1038/srep25729

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(A) HCV genome sequence corresponding to the C + HV fragment containing the CRE structure and the downstream region for the hybridization of the oligonucleotide used in the pull-down experiments (red). (B) Proteomic method for identifying CRE-interacting candidates. Binding reactions between Huh-7 S10 lysates and CRE were incubated in the presence of streptavidin-coated magnetic beads (Dynabeads) and pulled-down. Some 1/3 of the sample was subjected to SDS-PAGE and the protein stained. Line 1: control samples corresponding to CRE linked to non-biotinylated asHCV-9414; Line 2: positive samples corresponding to CRE linked to biotinylated asHCV-9414 (b-asHCV-9414). Some 2/3 of the samples were digested, analyzed by LC-MS/MS, and compared two by two as explained in Methods.