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. 2016 May 11;6:25729. doi: 10.1038/srep25729

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Copyright © 2016, Macmillan Publishers Limited

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(A) Scheme of the ICU and Cap-RLuc RNA constructs. ICU is composed by HCV IRES, CRE and 3′UTR regions flanking the reporter gene of the Firefly luciferase. cap-RLuc contains Renilla luciferase RNA, capped during in vitro transcription. (B) Huh-7 cells were treated with the indicated siRNAs and transfected with a mix containing 1 μg FLuc-ICU and 0.25 μg cap-RLuc per well. Luciferase activity was measured after 4 h and translation efficiency calculated as FLuc/RLuc activities. Standard deviation is displayed for each siRNA. Significant differences between siRNAs and non-targeting siRNA are indicated above the corresponding bar (p < 0.05 in a two-tailed Student’s t-test).