Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 11;6:25765. doi: 10.1038/srep25765

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

EndoC-βH2 cells (a) and adult human islets (b) were transfected with control non-target siRNA (siNT) or siRNA targeting RFX6 (siRFX6). GPR68 expression was analyzed 72 h post transfection by RT-qPCR. Data are expressed as percentage of siNT transfected cells. (c–e) wtRFX6 and Mut506RFX6 (one-way ANOVA) (c), transactivation domain VP16- conjugated RFX6 (d) and transcriptional repression domain KRAB- conjugated RFX6 (e) were expressed in EndoC-βH2 cells using bicistronic constructs with IRES-EGFP. GFP^+ve cells were FACS isolated 48 h post-transfection and GPR68 expression was analyzed by RT-qPCR. Data are mean ± SEM of 3–5 experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns, not significant.