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. 2016 May 11;6:25765. doi: 10.1038/srep25765

Figure 3. Extracellular pH modulates IP production but not cAMP accumulation in EndoC-βH2 cells.

Figure 3

(a) IP formation was determined in EndoC-βH2 cells incubated at pH 7.4 and 6.4. The Gq/11-selective inhibitor compound YM-254890 (100 nM) was also tested. Results are expressed as fold change over the IP values at pH 7.4. (b) For cAMP formation assay, EndoC-βH2 cells were incubated for 30 min with and without Forskolin (25 μM) at pH 7.4 or 6.4. Results are expressed as cAMP accumulation (nM) per 5,000 cells. (c) EndoC-βH2 cells were transfected with control siRNA (siNT), siRNA targeting either GPR68 (siGPR68) or RFX6 (siRFX6). 72 h post transfection, cells were incubated at pH 7.4 or 6.4 for 30 min and analyzed for the IP formation. Results are expressed as fold change over the IP values at pH 7.4. Data are mean ± SEM of 3–5 experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns, not significant (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).