Skip to main content
. 2016 May 11;6:25765. doi: 10.1038/srep25765

Figure 5. IL-8 induction upon extracellular acidification is RFX6- and GPR68-dependent.

Figure 5

(a) EndoC-βH2 cells were cultured for 24 h at pH 7.4 or 6.4 with or without YM-254890 (100 nM), a Gq/11-selective inhibitor compound. IL-8 expression was analyzed by RT-qPCR. (b) EndoC-βH2 cells were transfected with siRNA (siNT), siGPR68 or siRFX6. After 48 h, cells were cultured for an additional 24 h period at pH 7.4 or 6.4 and next analyzed for the expression of IL-8 by RT-qPCR. Data are mean ± SEM of 3–5 experiments. **p < 0.01; ***p < 0.001 (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).