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. 2016 May 11;6:25765. doi: 10.1038/srep25765

Figure 7. IL-8 secreted by EndoC-βH2 cells in acidic conditions attracts neutrophils.

Figure 7

(a) Neutrophils were isolated from whole blood using MACSxpress kit, analyzed for the expression of CD16 and used for in-vitro transwell migration assay. (b) Neutrophil chemotaxis was tested using conditioned media from EndoC-βH2 cells cultured at pH 7.4 or pH 6.4 for 72 h. Acidic pH 6.4 conditioned medium pre-treated for 10 min with Anti-human IL-8 (1 μg/ml) was also used as well as pH 7.4 conditioned medium supplemented with recombinant human-IL-8 (50 ng/ml). Data are represented as migration index, calculated by assigning a value of 1 to the number of migrating neutrophils towards pH 7.4-conditioned medium. Data are mean ± SEM of 4 blood donors. *p < 0.05; **p < 0.01 (one-way ANOVA, followed by a Tukey’s multiple comparisons post-test).