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Live/dead (green/red) staining of NRVM after 8, 14, and 21 days of culture in different conditions: Top-row panels, 2D tissue culture plate coated with gelatin (control). Middle-row panels, NRVM cultured in 3D PSHU-PNIPAAm-lysine. Bottom-row panels: NRMV cultured in 3D PSHU-PNIPAAm. Compared to control groups, NRVM cultured in both 3D matrices showed a well-developed cell-interconnected network that may allow better impulse propagation between cells. Scale bar: 400 μm.
