Table 1. MRM transitions for orexin-A with collision energy and resolution.
| m/z precursor ion | Charge | MS1 Resolution | m/z product ion | Charge | Fragment | MS2 Resolution | Collision Energy (eV) |
|---|---|---|---|---|---|---|---|
| 891.2 (901.4) | 4+ | wide | 854.1 (862.4) | 4+ | b32-H2O | Wide | 22 |
| 713.3 (721.1) | 5+ | wide | 858.6 (866.9) | 4+ | b29 | Wide | 5 |
| 713.3 (721.1) | 5+ | wide | 854.1 (862.4) | 4+ | b32-H2O | Wide | 12 |
| 713.3 (721.1) | 5+ | wide | 683.9 (690.2) | 5+ | b32-H2O | Wide | 8 |
Corresponding data on the SIS are given in brackets. The transition used as the orexin-A quantifier is presented in bold print.