Table 2. Summary of LC-MS/MS conditions.

Column	Zorbax 300 SB-C18 (3.5 um, 1.0 × 150 mm i.d.)
Mobile phase A	0.1% Formic Acid/3% acetonitrile/96.9% water
Mobile phase B	0.1% Formic Acid/99.9% acetonitrile
	25–38% B for 3.8 min
Linear gradient	100% B for 1 min
	0% B for 4 min
Flow rate	100 μL/min
Injection Volume	20 μL in the full loop mode
Column temperature	50 °C
Polarity	Positive ESI
Capillary voltage	3000 Volts
Nebulizer	45 psi
Gas flow	12 L/min
Gas flow temperature	150 °C
Sheath gas flow rate	7 L/min
Sheath gas temperature	250 °C
High pressure ion funnel RF	150 V
Low pressure ion funnel RF	110 V
Cell Accelerator Voltage	6 V
Collision-induced dissociation gas	Nitrogen