Figure 2.

RACE PCR to attempt the amplification of FmFLS2-2. (a) Using ‘Nagami’ kumquat cDNA as template a forward primer (RACE3ʹ-f, Supplementary Table 1) was used in combination with the universal reverse primer (GeneRacer 3ʹ, Supplementary Table 1), which hybridizes to the poly-A tail at the 3ʹ end of the messenger RNA. (b) Schematic representation of the theoretical target versus the PCR product obtained. Top: based on the genomic map reported for C. sinensis and C. clementina (http://citrus.hzau.edu.cn/orange/ and Phytozome.org) the expected 1-kb-long amplicon would be composed of the 3ʹ protein-coding region of FLS2-2 and its corresponding 3′ UTR. Bottom: the actual amplicon, as revealed by sequencing, was a combination of the protein-coding region of FLS2-1 and the 3′ UTR of FLS2-2. cDNA, complementary DNA; UTR, untranslated region.