Figure 8.
Response to Xflg22 and citrus canker in ‘Duncan’ grapefruit leaves transiently expressing various citrus FLS2 plasmid constructs. (a) Leaves were treated with 10 μm Xflg22 or water as the control 3 days after infiltration with Agrobacterium containing the plasmids. The leaves were tested for Xflg22-responsive gene expression 24 h after the treatment. The constructs tested are indicated above each graphic. RQ is the relative quantification of gene expression 24 h after Xflg22 or water (control) treatment. An asterisk represents RQ values significantly different (P<0.05) from the water control. Bars are means of replicates±s.e. (n=3). (b) Xcc bacterial suspensions (5×105 c.f.u. per mL) were inoculated into leaves 1 day after the agroinfiltrations. The citrus canker symptoms were recorded 2 weeks after the inoculations. The plasmid constructs and controls used in the agroinfiltrations are indicated below. The experiments were repeated twice with similar results. c.f.u., colony-forming unit.