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. 2016 May 11;6:25520. doi: 10.1038/srep25520

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Copyright © 2016, Macmillan Publishers Limited

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(a) Schematic of experimental setup. Transwell system where macrophages (RAW264.7) were plated on recording electrodes (upper chamber) and mouse astrocyte conditioned media with or without 7α25HC were added to the lower chamber. The migration of macrophages from the upper chamber to the lower chamber was measured by the recording electrodes two hours after addition of astrocyte media or drugs to the bottom chamber. (b) Treatment with 7α25HC (0.01 μM, 2 hours) induced a significant increase in macrophage migration. Astrocyte media collected after 6 and 8 hours of LPS (100 ng/ml) treatment induced macrophage (RAW264.7) migration while media from non-treated astrocytes or treated with LPS for 0, 2, 4, and 24 hours did not induce macrophage migration. Data presented as mean +/− SEM, n = 3 − 5, one-way ANOVAs with Dunnett’s post hoc tests, *p < 0.05, ***p < 0.001 vs. corresponding control.