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. 2016 May 11;6:25520. doi: 10.1038/srep25520

Figure 4. Macrophage (RAW264.7) migration induced by conditioned media from LPS-treated mouse astrocytes is reduced by EBI2 antagonism.

Figure 4

Please see Fig. 1a for experimental setup. Both 7α25HC (0.01 μM) and astrocyte conditioned media obtained from 6 and 8 hour LPS-treated mouse astrocytes induced macrophage migration. Addition of the EBI2 antagonist (NIBR189, 10 μM) to 7α25HC or the LPS-treated mouse astrocyte media attenuated astrocyte conditioned media effects on macrophage migration. Data presented as mean +/− SEM n = 3, one-way ANOVAs with Bonferroni’s post-tests, *p < 0.05, vs. corresponding control.