Figure 10.

Suppression of the Mn²⁺-Sensitive Phenotype of the Δpmr1 Yeast Mutant via Expression of PAM71.

Cells of the yeast mutant in the P-type Ca²⁺/Mn²⁺-ATPase (Δpmr1) containing the empty vector pDR196 (+pDR196) or vector with PAM71 (+PAM71) were verified by PCR and dropped on synthetic medium (control) and on synthetic medium supplemented with 2 mM MnCl₂. For selection, uracil was added (+) or omitted (−). Undiluted cells (10°) corresponded to an OD₆₀₀ = 3.0. Photographs were taken after 4 d at 30°C.