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. 2016 Mar 28;28(4):892–910. doi: 10.1105/tpc.15.00812

Figure 4.

Figure 4.

Sequence Alignment of At-PAM71, At-PAM71-HL, and Its Homologs from Yeast and Human.

The protein sequence alignment was generated using Clustal Omega (version 1.2.1). Black and gray boxes indicate identical residues and conservative exchanges (scoring > 0.5), respectively. Six putative transmembrane spans were manually adjusted and numbered TM1 to TM6 using Hs-TMEM165 and Sc-GDT1 as references (Demaegd et al., 2013). An additional transmembrane domain predicted by TmHMM (version 2) is indicated by a dashed line. The two highly conserved internal E-x-G-D-(KR)-(TS) motifs are highlighted by red lines. The putative sites of cleavage of the N-terminal chloroplast transit peptide (cTP) of PAM71 and PAM71-HL, as predicted by TargetP (version1), are indicated by a green arrow and a light-green arrow, respectively. The central loop is indicated by a dotted line. NCBI accession codes are as follows: Homo sapiens NP_060945.2 (transmembrane protein 165, TMEM165); Saccharomyces cerevisiae AHY74663.1 (GDT1); Arabidopsis thaliana NP_564825.1 (At1g64150, PAM71); and Arabidopsis thaliana NP_193095.2 (At4g13590, PAM71-HL).