Figure 8.

Manganese Supplementation Restores Photosynthesis in pam71.

(A) Seeds of the wild type, pam71-1, psbo1 psbo2, pam71-2, and pam71-1_{Pro35S:PAM71-GFP} were surface sterilized and grown for 2 weeks on MS medium containing either 1× CaCl₂ (0.332 g L⁻¹) and 1× MnSO₄ (0.017 g L⁻¹), 10× CaCl₂ (3.32 g L⁻¹) and 1× MnSO₄, or 1× CaCl₂ and 10× MnSO₄ (0.17 g L⁻¹). At the end of the growth period, the maximum quantum yield of PSII (F_V/F_M) was recorded.

(B) F_V/F_M values are given for the wild type, pam71-1, psbo1 psbo2, pam71-2, and pam71-1_{Pro35S:PAM71-GFP} grown on the indicated MS medium. Bars represent mean values (±sd) of three experiments, each including five individual plants (n = 15). Asterisks indicate statistical significance (t test, P < 0.001) of differences between wild-type and mutant plants.