Figure 10.

Both the N and C Termini of SLAC1 Are Capable of Interacting with and Inhibiting KAT1.

(A) Y2H data show protein-protein interaction between KAT1 and either SLAC1’s N terminus or SLAC1’s C terminus.

(B) to (F) Typical whole-oocyte recordings of oocytes injected with water (B) and the cRNA of KAT1 (C), KAT1 + full-length SLAC1 (D), KAT1+ SLAC1 N terminus (E), and KAT1 + SLAC1 C terminus (F).

(G) The average current-voltage curves of whole-oocyte currents of the oocytes injected with water (n = 5), the cRNA of KAT1 (n = 9), KAT1 + full-length SLAC1 (n = 7), KAT1 + SLAC1 N terminus (n = 9), and KAT1 + SLAC1 C terminus (n = 8).

(H) Normalized macroscopic Shaker conductance of the oocytes. Normalization was performed as described in Methods, and the numbers of oocytes tested were the same as described for (G). Error bars depict means ± se.