SLAC1-S456F and SLAC1-Δ1461-1671 Show No S-Type Anion Channel Activity.
(A) to (G) Typical whole-oocyte recordings of control oocytes injected with water (A) and oocytes injected with the cRNA of SLAC1 alone (B), SLAC1 + OST1
(C), SLAC1-S456F alone (D), SLAC1-S456F + OST1
(E), SLAC1-Δ1461-1671 alone (F), and SLAC1-Δ1461-1671 + OST1
(G). The ratios of cRNA mixture in (C) and (E) to (G) were 1:1.
(H) Average instantaneous SLAC1 currents recorded at −100 mV. The number of oocytes tested was 5 for the control, SLAC1, SLAC1 + OST1, SLAC1-S456F, SLAC1-S456F + OST1, and SLAC1-Δ1461-1671, and four for SLAC1-Δ1461-1671 + OST1. Error bars depict means ± se.