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. 2016 Mar 21;28(4):949–965. doi: 10.1105/tpc.15.01050

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© 2016 American Society of Plant Biologists. All rights reserved.

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Figure 8. — Loss-of-Function SLAC1-S456F and SLAC1-Δ1461-1671 Inhibit KAT1 Dramatically in X. laevis Oocytes.

(A) to (D) Typical whole-oocyte recordings of control oocytes injected with water (A) and oocytes expressing KAT1 alone (B), KAT1 + SLAC1-S456F (C), and KAT1 + SLAC1-Δ1461-1671 (D). The [cRNA] ratios of KAT1/SLAC1-S456F in (C) and KAT1/SLAC1-Δ1461-1671 in (D) were 1:2.

(E) Average current-voltage curves of whole-oocyte currents recorded in oocytes injected with water (n = 5), KAT1 cRNA (n = 7), a cRNA mixture of KAT1 + SLAC1-S456F (n = 6), and a cRNA mixture of KAT1 + SLAC1-Δ1461-1671 (n = 10).

(F) Relative macroscopic Shaker conductance and immunoblot analysis. The oocyte numbers for K⁺_in recordings in (F) were the same as described for (E). Error bars depict means ± se.