Figure 4C.

Original: RNase Protection Assay.

Total RNAs from wild-type or rap-1 plants were hybridized with the respective radiolabeled probe indicated below the panel (cf. [A]) and treated with single-strand specific RNases A and T1. Protected fragments were analyzed on a sequencing gel alongside 1/30 of the respective undigested hybridization probe (probe). Probes incubated with yeast tRNA before RNase digestion (lane “tRNA”) were used as a control. Black arrows mark fragments that are less abundant in rap-1 and asterisks major fragments protected in both the wild type and rap-1. Expected sizes of fragments were estimated from the running fronts of xylene cyanol (∼40 nucleotides) and bromophenol blue (∼15 nucleotides) indicated on the right.