Figure 5.

Original: rRAP Exhibits an Intrinsic RNA Binding Capacity.

(A) Purification of rRAP protein. Coomassie blue–stained SDS-PAGE gel showing the affinity-purified rRAP protein after removal of the maltose binding protein tag that was electrophoresed alongside authentic MBP. Mobilities of size markers are indicated on the left. Note that the two samples were electrophoresed on the same gel but not in adjacent lanes.

(B) UV cross-linking experiment. Purified rRAP protein, together with two control proteins (MBP and the RNA binding protein RBP40), was analyzed after UV cross-linking in the presence of a radiolabeled RNA probe corresponding to the 16S region spanning FP1 (pre-16S 5′ region). Sizes of marker bands are given in kilodaltons on the left.