Loss of surface CD26 correlates with disease stage and loss of intracellular IL-16.
A) Loss of surface CD26 was determined by flow cytometry. Cells were gated on CD3 then analyzed for CD26 expression. Number of donors: normal = 4, stage IA = 2, stage IA/B = 3, stage IB = 6, stage II = 2, stage III = 2, stage IV = 5.
B) Demonstration of concordant loss of both intracellular IL-16 and surface CD26. Together with disease stage, we observed losses in both intracellular IL-16 and surface CD26. Quadrant gates were created based on isotype controls from individual experiments.
C) Correlation of loss of intracellular IL-16 and surface CD26 (correlation coefficient = 0.403; P=0.002). Number of donors: normal = 5, stage IA = 2, stage IA/B = 3, stage IB = 6, stage II = 1, stage III = 2, stage IV = 5.