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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Contraception. 2015 Nov 14;93(4):337–346. doi: 10.1016/j.contraception.2015.11.008

Figure 4.

Figure 4

Cytotoxicity of VFS and SFS buffers in HEC-1-A cells. Naïve cells and N9 (0.1 mg/mL; N = 3) were used as controls and effect of exposure time (4, 8 and 24 h) and dilution with cell culture media followed by 24 h dosing was studied (N = 5, Mean ±SD). N9 (p < 0.001; unpaired t-test with two-tailed distribution and unequal variance) and undiluted VF compositions (p < 0.001; single factor ANOVA) showed significant toxicity with respect to no treatment. An increase in viability with VFS compositions diluted with cell culture media in the ratio 1:2, 1:10 and 1:20 was noted. Whole SFS-O was not toxic and diluted SFS−/+F (1:10 and 1:20) showed enhanced cell viability with no significant difference from untreated cells (p > 0.05; single factor ANOVA).