Table 1.
Compositions of Owen’s recipe (VFS-O) and proposed vaginal fluid simulants with (VFS+G) and without glucose (VFS-G)
| Chemical Name |
Formula | MW (g/mol) |
CAS # | Company | Grade/ Purity |
VFS-O | VFS+G | VFS-G | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1Mass(g) | Conc (mM) |
2Mass (g) |
3Conc. (mM) |
2Mass (g) |
3Conc. (mM) |
||||||
| D-glucose monohydrate |
C6H12O6.H2O | 180.1 | 14431-43-7 | Alfa Aesar |
99% | 5 | 27.8 | 5 | 25.2 | - | - |
| Sodium chloride |
NaCl | 58.44 | 7647-14-5 | Spectrum Chemicals |
USP | 3.51 | 60.5 | 4.5 | 77.6 | 5.25 | 90.6 |
| L(+)-Lactic acid |
C3H6O3 | 90.08 | 79-33-4 | Acros Organics |
90% | 42 | 26.3 | 40.61 | 8 | 40.61 | 8 |
| Potassium hydroxide |
KOH | 56.11 | 1310-58-3 | J.T.Baker | ACS | 1.4 | 25 | ||||
| Glacial acetic acid |
CH3CO2H | 60.05 | 64-19-7 | Alfa Aesar |
ACS/99.7% | 1 | 16.7 | 0.79 | 13.2 | 0.79 | 13.2 |
| Urea | NH2CONH2 | 69.06 | 57-13-6 | J.T.Baker | Ultrapure Bioreagent |
0.4 | 6.8 | - | - | - | - |
| Calcium hydroxide |
Ca(OH)2 | 74.09 | 1305-62-0 | J.T.Baker | ACS | 0.22 | 3 | - | - | - | - |
| Glycerol | C3H8O3 | 92.10 | 56-81-5 | Spectrum | USP | 0.16 | 1.7 | - | - | - | - |
| Bovine serum albumin (Fraction V) |
9048-46-8 | OmniPur | 0.018 | - | - | - | - | ||||
| DL-lactic acid sodium salt |
C3H5NaO3 | 112.06 | 72-17-3 | Fluka Analytical |
50 wt% aqueous solution |
- | - | 52.02 | 18 | 52.02 | 18 |
| Sodium acetate (anhydrous) |
C2H3NaO2 | 82.03 | 127-09-3 | Spectrum Chemicals |
USP | - | - | 0.31 | 3.8 | 0.31 | 3.8 |
(MW = molecular weight)
1
Mass of species to prepare 1L of 1X buffer
2
Mass of species to prepare 100 mL of 10X buffer
3
Concentration of the species in 1X buffer
4
Final mass of lactic acid and
5
sodium lactate to be added for buffer preparation.