Table 2.
Compositions of Owen’s recipe (SFS-O) and proposed seminal fluid simulants with (SFS+F) and without fructose and protein (SFS-F)
| Chemical Name |
Formula | MW (gmol) |
CAS # | Company | Grade | SFS-O | SFS+F | SFS-F | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1Mass (g) |
3Conc. (mM) |
2Mass (g) | Conc. (mM) |
2Mass (g) |
3Conc. (mM) |
||||||
| Sodium phosphate monobasic monohydrate |
NaH2PO4.H2O | 137.99 | 10049-21-5 | Mallinckrodt Chemicals |
USP-FCC | 0.92 | 6.72 | 0.97 | 7.08 | 0.97 | 7.08 |
| Sodium phosphate dibasic |
Na2HPO4 | 141.96 | 7558-79-4 | J.T. Baker | USP-FCC | 8.58 | 60.44 | 9.46 | 66.64 | 9.46 | 66.64 |
| Sodium citrate dihydrate |
C6H5Na3O7 | 294.1 | 6132-04-3 | J.T. Baker | USP-FCC | 8.13 | 27.64 | - | - | - | - |
| Potassium chloride |
KCl | 74.55 | 7447-40-7 | BDH | ACS | 0.908 | 12.18 | - | - | - | - |
| Potassium hydroxide |
KOH | 56.11 | 1310-58-3 | J.T. Baker | ACS | 0.88 | 15.74 | - | - | - | - |
| D-(-)- Fructose |
C6H12O6 | 180.16 | 57-48-7 | J.T. Baker | 2.72 | 15.1 | 3.64 | 20.24 | - | - | |
| D-Glucose monohydrate |
C6H12O6.H2O | 180.1 | 14431-43-7 | Alfa Aesar | 99% | 1.02 | 5.15 | - | - | - | - |
| L(+)-Lactic acid |
C3H6O3 | 90.08 | 79-33-4 | Acros Organics |
90% | 40.62 | 8.15 | 40.62 | 8.15 | 40.62 | 8.15 |
| Urea | NH2CONH2 | 69.06 | 57-13-6 | J.T.Baker | Ultrapure Bioreagent |
0.45 | 7.49 | - | - | - | - |
| Bovine serum albumin (Fraction V) |
9048-46-8 | OmniPur | 50.4 | 0.76 | 50.4 | 0.76 | - | - | |||
| Calcium chloride dehydrate |
CaCl2.2H2O | 147.02 | 10035-04-8 | Mallinckrodt Chemicals |
1.01 | 9.1 | - | - | - | - | |
| Magnesium chloride hexahydrate |
MgCl2.6H2O | 203.3 | 7791-18-6 | Mallinckrodt Chemicals |
0.92 | 4.53 | - | - | - | - | |
| Zinc chloride | ZnCl2 | 136.29 | 7646-85-7 | Acros Organics |
98+% | 0.34 | 2.5 | - | - | - | - |
| Sodium chloride |
NaCl | 58.44 | 7647-14-5 | Spectrum Chemicals |
USP | - | - | - | - | 0.46 | 8 |
(MW = molecular weight)
1
Mass of species to prepare 1L of 1X buffer
2
Mass of species to prepare 100 mL of 10X buffer
3
Concentration of the species in 1X buffer
4
Final mass of lactic acid to be added for buffer preparation