| Final concentration | |
|---|---|
| Tris-HCl (pH 8.0) | 10 mM |
| MgCl2 | 1 mM |
| EDTA | 1 mM |
| NP-40 | 1% (v/v) |
| Sodium deoxycholate | 0.25 mM |
| Glycerol | 10% (v/v) |
| NaCl | 150 mM |
| 2-mercaptoethanol | 5 mM |
| AEBSF | 0.1 mM |
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