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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Mol Microbiol. 2016 Feb 19;100(3):510–526. doi: 10.1111/mmi.13332

Figure 2.

Figure 2

Hyper-secretion of ESX-5 substrates by the ΔpstA1 mutant requires RegX3. Wild-type M. tuberculosis Erdman (WT), ΔpstA1, ΔregX3, ΔpstA1ΔregX3, ΔregX3 pNDregX3 and ΔpstA1ΔregX3 pNDregX3 were cultured in Sauton's complete medium without Tween-80 for 5 days. 10 μg of cell lysate (CL) and 4 μg of culture filtrate (CF) proteins were subjected to SDS-PAGE and Western blot analysis. Antibodies used are indicated. Anti-GroEL2 was used as both a loading control for the CL fraction and a cell lysis control in the CF fraction. Anti-ModD was used as a loading control for the CF fraction. Results shown are from a single experiment and are representative of 3 independent experiments.