Figure 2.
Hyper-secretion of ESX-5 substrates by the ΔpstA1 mutant requires RegX3. Wild-type M. tuberculosis Erdman (WT), ΔpstA1, ΔregX3, ΔpstA1ΔregX3, ΔregX3 pNDregX3 and ΔpstA1ΔregX3 pNDregX3 were cultured in Sauton's complete medium without Tween-80 for 5 days. 10 μg of cell lysate (CL) and 4 μg of culture filtrate (CF) proteins were subjected to SDS-PAGE and Western blot analysis. Antibodies used are indicated. Anti-GroEL2 was used as both a loading control for the CL fraction and a cell lysis control in the CF fraction. Anti-ModD was used as a loading control for the CF fraction. Results shown are from a single experiment and are representative of 3 independent experiments.