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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Mol Microbiol. 2016 Feb 19;100(3):510–526. doi: 10.1111/mmi.13332

Figure 6.

Figure 6

RegX3 is required for induction of ESX-5 protein secretion in response to phosphate limitation.

Wild-type M. tuberculosis Erdman (WT), ΔregX3, and ΔregX3 pNDregX3 strains were cultured for 5 days either in Sauton's complete medium without Tween-80 (control) or in Pi-free Sauton's medium without Tween-80 to which 2.5 μM KH2PO4 was added exogenously. 10 μg of cell lysate (CL) and 4 μg of culture filtrate (CF) proteins were subjected to SDS-PAGE and Western blot analysis. Anti-GroEL2 was used as both a loading control for the CL fraction and a cell lysis control in the CF fraction (data not shown). Anti-ModD was used as a loading control for the CF fraction. Results shown are representative of 3 independent experiments.