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. 2016 May 30;265:81–88. doi: 10.1016/j.jneumeth.2015.09.033
TRIS base (Sigma-Aldrich, no. T6066, USA) 48 g
Sodium chloride (Sigma-Aldrich, no. 71376, USA) 36 g
Distilled water 1000 ml
Adjusted to pH7.3 with concentrated HCl and stored at +4 °C