Figure 3.

(A–C) Ouabain (Oua) modulates mCD14 expression only in monocytes after 2-hour adhesion. Monocytes were allowed to attach to the substrate for either 2 hours or 24 hours. After that, cultures were washed for lymphocyte removal, and monocytes were then incubated with 100 nM Oua for a further 24 hours or left untreated. Next, monocytes were incubated with antihuman CD14, as described in Materials and methods. (A) Medians of mCD14^high cell percentages, denoting cells with a high expression of mCD14 in their surface; *difference from the respective control (Ctrl) (P < 0.01, Mann–Whitney test). (B) Variation of the means of fluorescence intensities (MFI) of mCD14 expression from monocytes incubated with Oua after 2-hour or 24-hour adhesion, compared with the Ctrl (set as 1); *statistically different from Ctrl (P < 0.0005, paired t-test). (C) Representative experiment showing the decrease in mCD14^high cells (gate located in the right) after treatment with Oua. These experiments were performed using six individuals.