Figure 8.

(A–D) Ouabain fails to induce CD69 expression in monocytes after 24-hour adhesion. Monocytes were maintained for adhesion in culture for 2 hours (A and B) or 24 hours (C and D), and then incubated with 100 nM Oua for 24 hours or left untreated. After that, monocytes were incubated with antihuman CD69, as described in Materials and methods.

Notes: (A and C) Means of CD69-positive cells in total population; *statistical difference from control (Ctrl) (P < 0.001, Mann–Whitney test). (B and D) Medians of CD69-positive cells in small and large monocytes; ^a,bstatistical difference from small and large monocyte Ctrl, respectively (^aP < 0.01 and ^bP < 0.05, Mann–Whitney test). These experiments were performed using six individuals.