Figure 9.

Role of p38MAPK inhibition in the modulation of monocyte cell-size progression induced by ouabain (Oua).

Notes: Monocytes were maintained for adhesion in culture for 2 hours and then incubated with 100 nM Oua for 24 hours, in the presence or absence of the p38MAPK inhibitor SB202190 (used at 20 µM). Values refer to the medians of large monocytes in each condition (evaluating only CD14⁺ cells); ^astatistical difference from control (P < 0.01, paired t-test); ^bstatistical difference from Oua + SB202190 (P < 0.05, paired t-test). Inset: Representative experiment showing monocyte cell-size and cell-granularity profiles obtained by flow cytometry, where gates delimiting small and large subpopulations are depicted, as well as both Oua and SB202190 effects on large-monocyte subpopulation number. These experiments were performed using six individuals.