Figure 1.
Profiling of miR‐182 expression in stimulated B cells. Real‐time RT‐PCR analyses indicate that the microRNA miR‐182 is preferentially expressed in activated B cells. Purified wild‐type and miR‐182 knockout (KO) B cells were stimulated with anti‐IgM (F(ab′)2) alone (10 μg/ml), anti‐CD40 alone (1 μg/ml), anti‐IgM (F(ab′)2) (10 μg/ml) and anti‐CD40 (1 μg/ml) antibodies, or lipopolysaccharide (LPS) alone (20 μg/ml) for 1–3 days. Cells were harvested and miR‐182 expression was determined by real‐time RT‐PCR, normalized to U6 snRNA expression. Data shown represent averages with standard deviations from three independent samples.