Figure 4.

Rapid CCR4⁺ regulatory T (Treg) cell accumulation in spleen in response to apoptotic cell injection. (a) Splenocytes from control (None) and apoptotic‐cell‐challenged mice (Apo) were stained with antibodies for CD4, CD8, CD11c, CD11b together with antibody for CCR4, respectively, and the percentages of CD4⁺ CCR4⁺ cells, CD8⁺ CCR4⁺ cells, CD11c⁺ CCR4⁺ cells and CD11b⁺ CCR4⁺ cells were analysed, respectively, by FACS. (b) Splenocytes from control (None) and apoptotic‐cell‐challenged (Apo) mice were also stained with antibodies for CD4, CD25 and CCR4 for flow cytometry analysis, and the percentage of CCR4 in CD4⁺ CD25^hi were compared. (c) Splenocytes from apoptotic‐cell‐challenged mice were applied in the upper chamber of the transwell, supplemented or not supplemented with CCL22. After 3 hr, the migrated cells in the lower chamber were stained with antibodies for CD4, CD25 and CCR4 for flow cytometry analysis, and the percentages of CCR4 in CD4⁺ CD25^hi were compared. *P < 0·05, **P < 0·01 compared with control, respectively.