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RbdB is critical for full processing of SrbA. Whole-cell extracts from the WT, ΔrbdB, ΔrbdB-R, SrbA1-425ΔrbdB, and ΔsrbA strains grown under normoxic and hypoxic conditions were subjected to immunoblot analysis with antibodies against amino acids 1 to 275 of SrbA (A) and against γ-tubulin (B). P, precursor form of SrbA; N, cleaved and active form of SrbA.
