FIG 1 .
TGME49_210370 is a novel rhoptry protein. (A) The cell cycle expression profile of TGME49_210370 is similar to known Toxoplasma effectors. (B) Illustration of TGME49_210370 with an HA tag at its endogenous locus. (C) IFA results showing HA-tagged TGME49_210370 colocalizes with ROP13 in the rhoptries. TGME49_210370 was thus designated ROP54HA. (D) Western blot analysis demonstrated ROP54 migrates as a doublet at its predicted size (53.6 kDa). (E) Results of the evacuole assay, demonstrating that ROP54HAII is secreted into the host cell, similar to the known rhoptry protein ROP13.