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. 2016 Mar 23;1(2):e00045-16. doi: 10.1128/mSphere.00045-16

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Copyright © 2016 Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Selective permeabilization demonstrated that ROP54 localizes to the PVM. (A) Digitonin permeabilization of HFFs infected with ROP54HA_II parasites for 12 h showed that ROP54 is present on the cytoplasmic face of the PVM (arrow). Overpermeabilized vacuoles were SAG1 positive and are annotated with an asterisk. (B) ROP5 control for vacuolar membrane localization under digitonin treatment conditions. (C and D) HFF monolayers were infected with ROP54HA_II parasites and then fixed and selectively permeabilized with digitonin 1 h postinfection (C) or 12 h postinfection (D). Whereas ROP5 localized relatively early on the PVM, ROP54 was more frequently found at later time points.