TABLE 2 .
Locations of Tn5 transposon gene insertions in grazing-susceptible mutantsd
| Grazing phenotype and locusa | Gene | Predicted function | Swarming | Mutant designation(s) |
|---|---|---|---|---|
| Grazing susceptible | ||||
| L585_12500 | ompR | Transcriptional regulator | Y | 28H12 |
| L585_05285 | cpxA | Histidine kinase | Y | 35D8, 18A11 |
| L585_21390 | phzR/luxR Chemical Formula: sdiA |
[Intergenic space-upstream] transcriptional regulator | Y | 10E5 |
| L585_04860 | ptsN | PTS sugar transporter subunit IIA | Y | 19E7 |
| L585_22810 | nagC/nagD | Transcriptional regulator (polar mutation of nagC) | Y | 26A5 |
| L585_16630 | dsbB | Disulfide bond formation protein | Y | 26H9 |
| L585_21750 | serC | 3-Phosphoserine/ phosphohydroxythreonine aminotransferase | Y | 28G12 |
| L585_20040 | srmB | RNA helicase | N | 36D8 |
| L585_10945 | yagG | [Intergenic space-upstream] uncharacterized member of the GPH family of galactose-pentose-hexuronide transporters (symporter) | Y | 31C1 |
| L585_00065 | motA | Flagellar motor protein | N | 36E1 |
| L585_00075 | flhD | [Intergenic space-upstream] transcriptional regulator and flagellar apparatus operon | N | 42A7, 42H1b |
| L585_10950 | eanI | Acylhomoserine lactone synthase | Y | 22A12 |
| Enhanced grazing susceptible | ||||
| L585_10305 | Hypothetical protein (rrf2 domain, badM-like transcriptional regulator) | Y | 16A5, 16A2,b 16A1,b 16H12b | |
| L585_04880 | arcB | [Intergenic space-downstream] aerobic respiration control sensor protein | Y | 24A2, 24A5c |
| L585_16795 | prc | Carboxy-terminal protease | Y | 14G9 |
| L585_21810 | trxB | Thioredoxin reductase | Y | 39D5 |
| L585_03130 | rhlB | Rhamnosyltransferase I, subunit B | Y | 32C5, 24H12, 25E4, 40C2 |
| L585_21385 | phzI | Autoinducer synthase | Y | 25E1, 26F4b |
| L585_03125 | rhlA | Rhamnosyltransferase I, subunit A | N | 28C8 |
| L585_04515 | pyrB | Aspartate carbamoyltransferase catalytic subunit | Y | 36A4 |
| L585_03535 | carB | Carbamoyl phosphate synthase large subunit | Y | 24G12 |
| L585_19770 | guaB | IMP dehydrogenase | Y | 4H11 |
| L585_21595 | pyrD | Dihydroorotate dehydrogenase | Y | 28H8 |
| L585_04660 | pnp | Polynucleotide phosphorylase/ polyadenylase | N | 30D8 |
| L585_15360 | prfC | Peptide chain release factor 3 | N | 8F6 |
| L585_21170 | pyrC | Dihydroorotase | Y | 35F10 |
a
Mutants exhibiting plaque formation were scored as grazing susceptible, whereas mutants in which 4 out of 8 test wells exhibited spore formation were classified as enhanced grazing susceptible. The NCBI accession number for the draft annotated whole-genome shotgun sequence is ASJH01000000.
b
Mutant not tested for swarming capability.
c
Mutant did not swarm.
d
Abbreviations: PTS, phosphotransferase; Y, yes; N, no.