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Sensitivity of ΔpprA mutant to gyrase inhibitors. Serial dilutions of cultures of GY9613 (wild-type), GY14661 (ΔpprA), GY15987 (ΔtopIB) (2 clones, a and b), and GY15985 (ΔpprA ΔtopIB) (2 clones, a and b) strains were spotted on plates in the presence or absence of novobiocin or nalidixic acid at the indicated concentrations, and plates were incubated at 30°C for 5 days.
