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PprA and DdrB are induced, whereas the DdrO concentration decreases after novobiocin treatment. D. radiodurans GY14615 (pprA::HA), GY12830 (ddrB::SPA), and GY16173 (ddrO::FLAG) cells were incubated in the presence or absence of novobiocin for the indicated times. Cell extracts were subjected to SDS-PAGE and analyzed by Western blotting with anti-HA antibodies for PprA::HA detection and anti-FLAG antibodies for DdrB::SPA and DdrO::FLAG detection.
