FIG 6 .

PprA stimulates decatenation activity of D. radiodurans DNA gyrase. (A) kDNA was incubated with the indicated increasing concentrations of DNA gyrase. kDNA was retained in the wells of the agarose gel. (B) Effect of PprA on the decatenation activity of DNA gyrase. DNA gyrase (4 nM) was incubated with kDNA in the presence of the indicated increasing concentrations of PprA. (C) Stimulation of DNA decatenation by PprA. The ratio of the minicircles released in the presence of PprA over the minicircles released in the absence of PprA was determined at each PprA concentration, and the average results from four independent experiments were plotted. SC, supercoiled minicircles; MC, minicircles; CI, catenated intermediates.