TABLE 2 .
Bacterial strains
| Bacterial strain | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU(ϕ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory stock |
| Rosetta 2(DE3)pLysS | F− ompT hsdSB(rB− mB−) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam-7 nin-5])pLysSRARE (cat) | Novagen |
| D. radiodurans strains | ||
| R1 | Wild type, ATCC 13939 | Laboratory stock |
| GY12830 | ddrB::SPA::cat | 13 |
| GY13338 | gyrA::SPA::cat | 36 |
| GY14615 | pprA::HA::kan | 21 |
| GY14641 | ΔpprAΩkan gyrA::SPA::cat | This work |
| GY14661 | ΔpprAΩcat | This work |
| GY14697 | pprA::HA::kan gyrA::SPA::cat | This work |
| GY15985 | ΔtopIBΩkan ΔpprAΩcat | This work |
| GY15987 | ΔtopIBΩkan | This work |
| GY16173 | ddrO::FLAG::cat | 19 |
| GY16179 | pprA::6His::kan | This work |