FIG 4 .

The mig1Δ and hapXΔ mutants have altered susceptibilities to inhibitors of cellular respiration and reactive oxygen species. The results of spot assays are shown for serial dilutions of the WT and derivative mutants under the following conditions. (A) YPD plus inhibitors of electron transport chain complexes I to IV and the alternative oxidase (75 µg ⋅ ml⁻¹ rotenone, 1 mM malonic acid, 3 µg ⋅ ml⁻¹ antimycin A, 10 mM potassium cyanide [KCN], and 5 mM SHAM). (B) YPD plates plus ROS inducers (0.01% H₂O₂, 50 µM plumbagin, 5 µg ⋅ ml⁻¹ menadione, 500 µM paraquat, and 50 µM DPI). Plates were incubated at 30°C and 37°C for 2 days. (C) YES plus hypoxia-mimicking agent cobalt-chloride (600 µM CoCl₂). Plates were incubated at 30°C or 37°C for 2 days.