FIG 5 .

The mig1Δ mutant has enhanced susceptibility to fluconazole. (A) Spot assay of serially diluted WT cells of strain H99 and derivative mutants on YPD plates with 10 µg ⋅ ml⁻¹ fluconazole (Flu) and/or 100 µg ⋅ ml⁻¹ tetracycline (Tet). The plates were incubated at 30°C for 2 days. (B and C) Survival of WT strain H99 and derivative mig1Δ mutants grown in YPD liquid media with and without fluconazole and/or tetracycline at 30°C (B) or 37°C (C) with agitation. The numbers of cells per milliliter of each culture were counted at 0, 24, and 48 h, and the means ± SEM were calculated. The experiment was performed twice with biological triplicates. Statistical analysis was performed using an unpaired two-tailed t test, where P is <0.05 (*).