FIG 8 .

The hapXΔ mig1Δ mutant but not the mig1Δ mutant is attenuated for virulence in mice. Ten female BALB/c mice were infected intranasally with either the WT strain, the mig1Δ mutant, or the hapXΔ mig1Δ mutants, and survival of the mice was monitored over 22 days. (A) The difference in survival between the mig1Δ mutant-infected and the WT-infected mice was not significant, but a significant difference was statistically reached between values for mice infected with the WT and the hapXΔ mig1Δ(A) mutant (P < 0.05) and between values for mice infected with the mig1Δ, hapXΔ mig1Δ(A), and hapXΔ mig1Δ(B) mutants (P < 0.0001) based on the log-rank Mantel-Cox test. (B) Fungal burden was determined in systemic organs and cardiac blood for three mice infected with the WT strain, the mig1Δ mutant, the hapXΔ mig1Δ mutants, or the mig1Δ::MIG1 mutant at the time of death (*, P < 0.05). The Mann-Whitney U test was used for statistical analysis.