FIG 2 .

K40 acetylation is dispensable in the presence of the T239I oryzalin resistance mutation. (A) Diagram of the TgTUBA1 genomic locus aligned with the allelic replacement construct containing the oryzalin resistance mutation T239I and the K40K, K40R, or K40Q mutation. (B) IFAs of mutant parasite lines stained for acetyl-K40–α-tubulin (red) or β-tubulin (green). Images were merged with the DNA stain DAPI (blue). Scale bars, 3 μm. (C) Western blot (WB) assay of parental RH and mutants confirms the loss of K40 acetylation in K40Q and K40R parasites. The blots were probed with anti-acetyl-K40–α-tubulin and anti-SAG1 antibodies as a loading control. (D) Doubling assays performed as described in the legend to Fig. 1D to assess the growth of parental RH and mutant parasites.