FIG 4 .
Expression of TgATAT and acetylated α-tubulin during the tachyzoite cell cycle. (A) Western blot (WB) assay of lysates from the parental strain (RHΔhxΔku80) and parasites containing endogenously HA-tagged TgATAT (TgATATHA). The blot was probed with antibodies recognizing the HA epitope or acetyl-K40–α-tubulin. β-Tubulin was also probed as a loading control. (B) IFAs of TgATATHA parasites stained for HA (red, TgATATHA) or β-tubulin (green) at the indicated stages of the parasite cell cycle. Images were merged with the DNA stain DAPI (blue). (C) IFAs of RH parasites stained for acetyl-K40–α-tubulin (red) or β-tubulin (green) at the indicated stages of the parasite cell cycle. Note that K40 acetylation is present on both spindle microtubules during mitosis and in the daughter subpellicular microtubules throughout replication. Scale bar, 3 μm.