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Rescue of E. coli CFT073 sdhA quiescence by fumarate. E. coli CFT073 sdhA was grown overnight in 0.4% glucose M9 minimal medium, and inocula of 105 CFU were seeded on 0.2% glucose plates containing disodium fumarate (200 µg/ml) (A) or 0.2% glucose plates (B). One hour later, 5 µl of a mixture of 1.0 mM lysine, 1.0 mM methionine, and 1.0 mM tyrosine was spotted to each plate, and the plates were incubated at 37°C for 24 h.
