TABLE 1 .
Bacterial strains
| E. coli strain | Genotype/phenotype | Designation in text | Source or reference |
|---|---|---|---|
| CFT073 Strr | Spontaneous streptomycin-resistant mutant of CFT073, has 5-bp duplication in rpoS | CFT073 | 53 |
| CFT073 Strr mini-Tn5 Km::gdhA | Mini-Tn5 Km glutamate dehydrogenase mutant of CFT073 Strr | CFT073 gdhA | This study |
| CFT073 Strr mini-Tn5 Km::gnd | Mini-Tn5 Km 6-phosphogluconate dehydrogenase mutant of CFT073 Strr | CFT073 gnd | This study |
| CFT073 Strr mini-Tn5 Km::pykF | Mini-Tn5 Km pyruvate kinase mutant of CFT073 Strr | CFT073 pykF | This study |
| CFT073 Strr mini-Tn5 Km::sdhA | Mini-Tn5::Km flavoprotein subunit of succinate dehydrogenase mutant of CFT073 Strr | CFT073 sdhA | This study |
| CFT073 Strr mini-Tn5 Km::zwf | Mini-Tn5::Km glucose-6-phosphate dehydrogenase mutant of CFT073 Strr | CFT073 zwf | This study |
| Wild-type CFT073 | Original clinical isolate | CFT073 original clinical isolate | 34 |
| Nissle 1917 Strr | Spontaneous streptomycin-resistant mutant of Nissle 1917 | Nissle 1917 | 57 |
| MG1655 Strr | Spontaneous streptomycin-resistant mutant of MG1655 | MG1655 | 54 |
| HS Strr | Spontaneous streptomycin-resistant mutant of HS | HS | 55 |
| EFC1 Strr | Spontaneous streptomycin-resistant mutant of EFC1 | EFC1 | 55 |
| EFC2 Strr | Spontaneous streptomycin-resistant mutant of EFC2 | EFC2 | 55 |
| F-18 Strr Nalr | Spontaneous streptomycin- and nalidixic acid-resistant mutant of F-18 | F-18 | 56 |
| EDL933 Strr | Spontaneous streptomycin-resistant mutant of EDL933 | EDL933 | 54 |
| ATM161 | Host for pUT, which contains the mini-Tn5 Km transposon (kanamycin resistance) | ATM161 | 17 |