BGHV8 v-OX2 clusters with gammaherpesviruses and Myotis sequences. (A) Representative alignment of BGHV8 OX2 with KSHV v-OX2 and Myotis davidii OX2. Dark red indicates identical residues, pink indicates conservation between two of the three alignments, and blue indicates less conserved regions. (B) Tree of representative viral OX2 ORFs demonstrates the relationship of BGHV8 v-OX2 to other gammaherpesviruses. Bold black denotes the penguinpox virus v-OX2 outgroup, black indicates gammaherpesvirus v-OX2, and pink denotes betaherpesvirus v-OX2. The red label highlights the location of BGHV8. (C) Tree of representative mammalian OX2 ORFs demonstrates the relatedness of BGHV8 OX2 to those from bat species. Blue labels denote microbat sequences, and green labels denote megabat sequences. The red label highlights the location of BGHV8.